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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
11/12/2003 |
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Data da última atualização: |
21/11/2005 |
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Autoria: |
GERMANO, M. G.; HUNGRIA, M. |
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Título: |
Ribosomal phylogeny of a brazilian collection of bradyrhizobium symbionts of thirty-three legume species. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 22., 2003, Florianópolis. [Resumos]. Florianópolis: Sociedade Brasileira de Microbiologia, 2003. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
Seção: Microbiologia de Solos. |
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Conteúdo: |
Many species of the family Leguminosae (Fabaceae) establish effective symbioses with N2-fixing bacteria in the genus Bradyrhizobium, which is relatively poorly studied despite wide geographic distribution and legume-host range. Genetic diversity seems to be greater in tropical than in temperate species of rhizobia, thus three ribosomal regions of a Brazilian culture collection of 119 strains of Bradyrhizobium, isolated from thirty-three legume species, representing nine tribes and all three subfamilies, were analyzed by RFLP-PCR. For the 16S rRNA gene, reference strains of B. japonicum fit into two major clusters, joined at a level of similarity of 50%, which included forty-seven strains, 90% of which were isolated from soybean, while two other clusters, joined at a similarity of 53%, were composed of strains of B. elkanii. Furthermore, three other major clusters were identified in which all strains were clustered at a final level of similarity of only 28%. For the intergenic spacer region (ITS), strains were clustered at a final level of similarity of 27%; reference strains of B. japonicum fit into a major group at 37% of similarity that included fifty-six strains, 84% isolated from soybean, while strains of B. elkanii fit into another major group, at a 44% level of similarity, clustering fifty-three strains, 64% isolated from other hosts than soybean. New clusters were also observed for the ITS region. The highest variability was detected in the analysis of the 23S rRNA gene with many distinct clusters and a very low level of similarity (16%) in the final grouping of the strains. A polyphasic approach, considering the three ribosomal regions, confirmed two great groups, related to B. japonicum and B. elkanii, at similarity levels of 54 and 46%, respectively. However, subclusters were defined within those two groups, and might be related to intraspecific variability, or to new subspecies, or even to new species. Furthermore, at least two new clusters were observed that might represent new species. The majority of strains isolated from soybean fit into the species B. japonicum, while most strains isolated from Brazilian legume species fit into B. elkanii or into the new clusters. The collection of bradyrhizobia analyzed in this study showed a genetic variability never reported before, confirming a high level of diversity of rhizobia in the tropics.
Partially financed by CNPq (PRONEX-and 520396/96-0). MenosMany species of the family Leguminosae (Fabaceae) establish effective symbioses with N2-fixing bacteria in the genus Bradyrhizobium, which is relatively poorly studied despite wide geographic distribution and legume-host range. Genetic diversity seems to be greater in tropical than in temperate species of rhizobia, thus three ribosomal regions of a Brazilian culture collection of 119 strains of Bradyrhizobium, isolated from thirty-three legume species, representing nine tribes and all three subfamilies, were analyzed by RFLP-PCR. For the 16S rRNA gene, reference strains of B. japonicum fit into two major clusters, joined at a level of similarity of 50%, which included forty-seven strains, 90% of which were isolated from soybean, while two other clusters, joined at a similarity of 53%, were composed of strains of B. elkanii. Furthermore, three other major clusters were identified in which all strains were clustered at a final level of similarity of only 28%. For the intergenic spacer region (ITS), strains were clustered at a final level of similarity of 27%; reference strains of B. japonicum fit into a major group at 37% of similarity that included fifty-six strains, 84% isolated from soybean, while strains of B. elkanii fit into another major group, at a 44% level of similarity, clustering fifty-three strains, 64% isolated from other hosts than soybean. New clusters were also observed for the ITS region. The highest variability was detected in the analysis of the 23S rRNA ge... Mostrar Tudo |
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Categoria do assunto: |
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LEADER 03017naa a2200157 a 4500
001 1465932
005 2005-11-21
008 2003 bl uuuu u00u1 u #d
100 1 $aGERMANO, M. G.
245 $aRibosomal phylogeny of a brazilian collection of bradyrhizobium symbionts of thirty-three legume species.
260 $c2003
300 $c1 CD-ROM.
500 $aSeção: Microbiologia de Solos.
520 $aMany species of the family Leguminosae (Fabaceae) establish effective symbioses with N2-fixing bacteria in the genus Bradyrhizobium, which is relatively poorly studied despite wide geographic distribution and legume-host range. Genetic diversity seems to be greater in tropical than in temperate species of rhizobia, thus three ribosomal regions of a Brazilian culture collection of 119 strains of Bradyrhizobium, isolated from thirty-three legume species, representing nine tribes and all three subfamilies, were analyzed by RFLP-PCR. For the 16S rRNA gene, reference strains of B. japonicum fit into two major clusters, joined at a level of similarity of 50%, which included forty-seven strains, 90% of which were isolated from soybean, while two other clusters, joined at a similarity of 53%, were composed of strains of B. elkanii. Furthermore, three other major clusters were identified in which all strains were clustered at a final level of similarity of only 28%. For the intergenic spacer region (ITS), strains were clustered at a final level of similarity of 27%; reference strains of B. japonicum fit into a major group at 37% of similarity that included fifty-six strains, 84% isolated from soybean, while strains of B. elkanii fit into another major group, at a 44% level of similarity, clustering fifty-three strains, 64% isolated from other hosts than soybean. New clusters were also observed for the ITS region. The highest variability was detected in the analysis of the 23S rRNA gene with many distinct clusters and a very low level of similarity (16%) in the final grouping of the strains. A polyphasic approach, considering the three ribosomal regions, confirmed two great groups, related to B. japonicum and B. elkanii, at similarity levels of 54 and 46%, respectively. However, subclusters were defined within those two groups, and might be related to intraspecific variability, or to new subspecies, or even to new species. Furthermore, at least two new clusters were observed that might represent new species. The majority of strains isolated from soybean fit into the species B. japonicum, while most strains isolated from Brazilian legume species fit into B. elkanii or into the new clusters. The collection of bradyrhizobia analyzed in this study showed a genetic variability never reported before, confirming a high level of diversity of rhizobia in the tropics. Partially financed by CNPq (PRONEX-and 520396/96-0).
700 1 $aHUNGRIA, M.
773 $tIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 22., 2003, Florianópolis. [Resumos]. Florianópolis: Sociedade Brasileira de Microbiologia, 2003.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 24 | |
| 5. |  | NARDO, A. E.; BARZAN, R. R.; GERMANO, M. G.; LEITE, R. S.; OLIVEIRA JUNIOR, A. Avaliação de métodos químicos para determinação de nitrogênio em amostras de grãos de soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 7., 2012, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2012. p. 11-18. (Embrapa Soja. Documentos, 333). Editado por Paula Gerón Saiz de Mello.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 8. | | GONÇALVES, M. S.; CORSINO, D. L. M.; KLEINERT, J. J.; GERMANO, M. G.; CASTRO, C. de; OLIVEIRA JUNIOR, A. de. Métodos para quantificação do teor disponível de K no solo. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 13., 2018, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2018. p. 53-59.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 9. | | GERMANO, M. G.; MENDES, L. W.; IWAI, S.; TEIXEIRA, W. G.; QUENSEN, J.; TIEDJE, J.; TSAI, S. M. Pirosequenciamento e qPCR revelam extensa diversidade funcional em solos de terra preta de indio da Amazônia e carvão pirogênico. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia, MG.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 10. | | EL KADRI, S.; GERMANO, M. G.; OLIVEIRA, F. A. de; CASTRO, C. de; OLIVEIRA JUNIOR, A. de. Calibração dos níveis críticos de potássio nas folhas de soja de cultivares com tipo de crescimento indeterminado em diferentes estádios de desenvolvimento. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. p. 193-195. (Embrapa Soja. Documentos, 339).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 11. | | BAZONI, C. H. V.; KLEINERT, J. J.; GERMANO, M. G.; LEITE, R. S.; OLIVEIRA JUNIOR, A. de. Determinação de matéria orgânica em amostras de solo: estudo comparativo de métodos. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 8., 2013, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2013. p. 65-69. (Embrapa Soja. Documentos, 339).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 12. | | HUNGRIA, M.; MENNA, P.; GERMANO, M. G.; CHUEIRE, L. M. O.; BANGEL, E. V.; CAMPO, R. J. Diversity of a Brazilian collection of rhizobial strains. In: WANG, Y. P.; LIN, M.; TIAN, Z. X.; ELMERICH, C.; NEWTON. W. E. (Ed). Biological nitrogen fixation, sustainable agriculture and the environment. Dordrecht: Springer, 2005. p. 413. Proceedingsof the 14th International Nitrogen Fixation Congress.| Biblioteca(s): Embrapa Soja. |
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| 13. | | GERMANO, M. G.; GALLI-TERASAWA, L. V.; CHUEIRE, L. M. de O.; HUNGRIA, M.; BANGEL, E. V.; CAMPO, R. J. Identificação de estirpes de Bradyrhizobium japonicum/ B. elkanii mais eficientes e competitivas para a cultura da soja e avaliação das respostas à reinoculação em áreas com populações estabelecidas distintas de Bradyrhizobium (04.2001.338-01). In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2002: microbiologia de solos. Londrina: Embrapa Soja, 2003. p. 42-59. (Embrapa Soja. Documentos, 216).| Biblioteca(s): Embrapa Soja. |
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| 14. | | ALVES, A. C. A. M.; GERMANO, M. G.; ANDRADE, F. A.; KLEINERT, J. J.; CASTRO, C. de; OLIVEIRA JUNIOR, A. de; OLIVEIRA, F. A. de. Acidez potencial estimada pelo método do pH SMP em solos da Fazenda Modelo da Embrapa em Ponta Grossa-PR. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 18., 2023, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2023. (Embrapa Soja. Documentos, 453). Regina Maria Villas Bôas de Campos Leite, Larissa Alexandra Cardoso Moraes, Kelly Catharin, Editoras Técnicas. p. 113-119.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 15. | | OLIVEIRA, S. C. A.; CORSINO, D. L. M.; KLEINERT, J. J.; GERMANO, M. G.; CASTRO, C. de; OLIVEIRA JUNIOR, A. de. Avaliação da forma de coleta de folhas para fins de diagnose nutricional da soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 13., 2018, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2018. p. 60-69.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 16. | | FARINE, E. L.; MOREIRA, D. A.; NARDO, A. E.; CAMARGO, I. S. P. de; JORDÃO, L. T.; GERMANO, M. G.; KLEINERT, J. J.; OLIVEIRA JUNIOR, A. Comparação de dois métodos para determinação do teor de carbono orgânico do solo. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 6., 2011, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2011. p. 28-30. (Embrapa Soja. Documentos 328). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo. Disponível em: .| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 17. | | GERMANO, M. G.; CANNAVAN, F. S.; MENDES, L. W.; LIMA, A. B.; TEIXEIRA, W. G.; PELLIZARI, V. H.; TSAI, S. M. Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia. Pesquisa Agropecuária Brasileira, v. 47, n. 5, p. 654-664, maio 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Solos. |
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| 18. | | HUNGRIA, M.; GERMANO, M. G.; MENNA, P.; GALLI-TERASAWA, L. V.; CHUEIRE, L. M. de O.; BANGEL, E. V.; MENDES, I. C.; CAMPO, R. J. Diversity of rhizobia in brazilian soils. In: INTERNATIONAL CONGRESS ON NITROGEN FIXATION, 14., Beijing. Program and abstract book... [S.l.: s.n., 2004]. p. 131.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 19. | | LIMA, A. B.; CANNAVAN, F. de S.; GERMANO, M. G.; DINI-ANDREOTE, F.; FRANCHINI, J. C.; PAULA, A. M. de; TEIXEIRA, W. G.; TSAI, S. M. Effects of vegetation and seasonality on bacterial communities in Amazonian dark earth and adjacent soils. African Journal of Microbiology Research, v. 9, n. 40, p. 2119-2134, Oct. 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Solos. |
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| 20. | | TONANI, V. H. V.; MACHADO, T. G. de J.; GERMANO, M. G. G.; KLEINERT, J. J.; OLIVEIRA JUNIOR, A. de; OLIVEIRA, F. A. de; CASTRO, C. de. Estimativa da acidez potencial pelo método do pH SMP em solos basálticos da Fazenda Experimental da Embrapa Soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 17., 2022, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2022. (Embrapa Soja. Documentos, 446). Regina Maria Villas Bôas de Campos Leite, Larissa Alexandra Cardoso Moraes, Kelly Catharin, Editoras Técnicas. p. 104-111.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 24 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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